Trenbolone Acetate has a double bond at carbons 9 and 11, which significantly raises its binding affinity to the androgen receptor, slows down its rate of metabolism and inhibits it from aromatizing. The result of these characteristics is that it is considered one of the most powerful anabolic steroids around. In addition to its hormone structure, Trenbolone Acetate also has the benefit of having a relatively small ester attached to it. This ester is attached in order to regulate the release time of the hormone after its injection. This controlled release gives Trenbolone Acetate an extended half-life of two days. However, Trenbolone Acetate could have a range of forty eight to almost seventy two hours. This means Trenbolone Acetate is a fairly fast acting steroid, and it also means that injections should happen somewhat frequently so as to stabilize blood levels. While Trenbolone Acetate carries with it some of the common characteristics of other steroids, there is one single characteristic that lifts it far above the pack, making it superior in terms of potency and power. Trenbolone Acetate enhances protein synthesis, which is essentially the building block of muscle growth.
The first block of steers was shipped for slaughter on june 28, the second block was shipped for slaughter on july 6, the third block was shipped for slaughter on july 13, and the fourth block was shipped for slaughter on july 27, 2000. On the day of shipment, each pen was weighed in multiple groups, and animals from a pen were maintained separately during transport and harvest. The order of weighing and shipping of each pen within block at the feedlot was the same order in which each pen was harvested. Any buller steers that were pulled after d 50 remained segregated in the buller pen until shipment for slaughter. Buller steers corresponding to each study pen were shipped for harvest on the same day as the study pen, but buller steers were weighed and remained segregated through the harvest process. Individual carcass data determined by USDA graders were obtained from the customer sheet provided by the harvest facility (IBP, Amarillo, TX) and included hot carcass weight, quality grade, and yield grade. Adjusted final BW was calculated by dividing hot carcass weight by the overall mean dressing percentage.
The development of a competitive microtitration plate enzyme-immunoassay for monitoring trenbolone application to animals is described. 'Bridge heterology' was achieved with a rabbit antibody raised against 17 beta-trenbolone-hemisuccinate-BSA and 17 alpha-trenbolone glucuronide-alkaline phosphatase as a tracer. The required 17 alpha-trenbolone glucuronide was prepared by application of trenbolone acetate to a calf following isolation from urine by three HPLC steps. The glucuronide was linked to alkaline phosphatase by the mixed anhydride procedure. The EIA was performed by coating affinity purified sheep IgG antirabbit IgG to the microtitration plate well followed by the addition of sample, tracer and hormone-specific antibody. The absolute detection limit was less than 1 pg; 50% relative binding at approximately 3 pg which is about 20 times superior to our RIA. The sample blanks of purified extracts from urine, bile, faeces, liver and muscle were similar in both methods (EIA and RIA) and much lower than required for a reliable detection of positive samples. Assay variations were always less than 15%. For the EIA, radiolabelled trenbolone, which is not commercially available, is not necessary and according to our experience the EIA is more practicable and economic.