L-Arg was analysed by using an Alltima HP HILIC column (Grace, Hesperia, CA) with 5 μm particle diameter (250 x mm). The following solutions were utilized for HPLC-ESI-MS analysis: (eluent A) aqueous % TFA and % acetic acid (v/v), (eluent B) acetonitrile containing % (v/v) TFA and % acetic acid. An isocratic elution was applied with 85% of eluent B for 6 minutes accordingly to Shin et al. [ 47 ] at a flow rate of mL/min. The T splitter delivered a flow-rate of ml/min toward the diode array detector and ml/min toward the ESI source. The photodiode array detector was set at 214 and 276 nm. Mass spectra were collected every 3 ms in the positive ion mode. The MS spray voltage was kV, and the capillary temperature was 235°C. MS spectra were collected in the range 50-220m/z. 100 μL of sample was injected into the HPLC-MS apparatus, corresponding to 67 μL of saliva.
I stand corrected, Heather! To me, it looked like one of those new-fangled digital thermometers that I don’t like, as I’ve wasted money on them and they don’t register my temp! I was taught to do basal temp testing on an old fashioned thermometer & didn’t realize there was a difference in different new ones! I’m guessing I’d find one of those online, eh, as my local drug stores are not great. Thanks for clearing that up for me. BTW, this morning I enjoyed reading your article on 15 things to do for my adrenal issues. THANKS AGAIN.
Not surprisingly, no elevated risk was found in the three studies listed at bottom, which looked at urine metabolite levels rather than blood THC. This confirms that urine testing has no bearing on driving impairment. Despite this fact, US Department of Transportation regulations force millions of commercial drivers to submit to random urine testing. The government has never produced convincing scientific evidence that this policy is necessary or effective to protect public safety. But they're the government, so they don't have to provide any evidence!